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    Cayman Chemical aea cayman chemical
    Aea Cayman Chemical, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aea+cayman+chemical/aea+cayman+chemical/pm40541800-249-52-53
    Average 90 stars, based on 1 article reviews
    aea cayman chemical - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Amino acid prodrug of capsaicin improves pharmacokinetic properties in the mouse brain and pancreas.
    Article Snippet: A set of 12 standards was prepared for analysis like the biological samples, with the following concentration ranges: 2–5 000 nM of PGE2 (Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin D2 (PGD2, Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin F2α (PGF2α, Tocris Bioscience, Bristol, UK), 0.1–240 nM of AEA (Cayman Chemical Company, Ann Arbor, MI, USA), 7–17 600 nM of 2-AG (Cayman Chemical Company, Ann Arbor, MI, USA), 0.4–1 000 nM of oleoylethanolamide (OEA, Sigma-Aldrich, Burlington, MA, USA), and 0.4–1 100 nM of palmitoylethanolamide (PEA, Sigma-Aldrich, Burlington, MA, USA).

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: AEA (Cayman Chemical) and [3H]AEA were dissolved in a 1:1:18 mixture of ethanol: emulphor: saline.

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: Briefly, 300 μg of regional homogenate, 500μg fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical, Ann Arbor, MI) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1-3H] (a gift from the National Institute on Drug Abuse) were incubated in Tris + 1mM EDTA pH 7.6 at 37°C in triplicate for fifteen minutes while shaking.

    Article Title: Indirect CB2 receptor and mediator-dependent stimulation of human whole-blood neutrophils by exogenous and endogenous cannabinoids.
    Article Snippet: Immunomodulatory effects of endogenous and exogenous cannabinoids have been investigated in numerous studies, mostly performed with isolated cells or transformed cell lines, but only sparse data exist on human polymorphonuclear neutrophils (PMNs).. We therefore investigated the respiratory burst reaction of human whole-blood PMNs under the influence of cannabinoids using flow cytometry.. In their natural whole-blood milieu, a CB2 receptor-dependent stimulation of the PMN respiratory burst was found at nanomolar concentrations of CP55 940 [( )-cis-3-[2-hydroxy-4-(1,1-dimethylheptyl)phenyl]-trans4-(3-hydroxypropyl)cyclohexanol] and methanandamide after a 3-h incubation period, whereas the short-living and rapidly hydrolyzed endogenous ligand anandamide did not alter the burst reaction of whole-blood PMNs under the same experimental conditions.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: AEA (Cayman Chemical), and [ 3 H]AEA were dissolved in a 1:1:18 mixture of ethanol/alkamus-620/saline.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: In brief, 300 μg of regional homogenate, 500 μg of fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1- 3 H] (a generous gift from the National Institute on Drug Abuse, Bethesda, MD) were incubated in Tris + 1 mM EDTA, pH 7.6 at 37°C in triplicate for 15 min while shaking.

    Control:

    Article Title: Amino acid prodrug of capsaicin improves pharmacokinetic properties in the mouse brain and pancreas.
    Article Snippet: A set of 12 standards was prepared for analysis like the biological samples, with the following concentration ranges: 2–5 000 nM of PGE2 (Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin D2 (PGD2, Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin F2α (PGF2α, Tocris Bioscience, Bristol, UK), 0.1–240 nM of AEA (Cayman Chemical Company, Ann Arbor, MI, USA), 7–17 600 nM of 2-AG (Cayman Chemical Company, Ann Arbor, MI, USA), 0.4–1 000 nM of oleoylethanolamide (OEA, Sigma-Aldrich, Burlington, MA, USA), and 0.4–1 100 nM of palmitoylethanolamide (PEA, Sigma-Aldrich, Burlington, MA, USA).

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: AEA (Cayman Chemical) and [3H]AEA were dissolved in a 1:1:18 mixture of ethanol: emulphor: saline.

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: Briefly, 300 μg of regional homogenate, 500μg fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical, Ann Arbor, MI) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1-3H] (a gift from the National Institute on Drug Abuse) were incubated in Tris + 1mM EDTA pH 7.6 at 37°C in triplicate for fifteen minutes while shaking.

    Article Title: Indirect CB2 receptor and mediator-dependent stimulation of human whole-blood neutrophils by exogenous and endogenous cannabinoids.
    Article Snippet: Immunomodulatory effects of endogenous and exogenous cannabinoids have been investigated in numerous studies, mostly performed with isolated cells or transformed cell lines, but only sparse data exist on human polymorphonuclear neutrophils (PMNs).. We therefore investigated the respiratory burst reaction of human whole-blood PMNs under the influence of cannabinoids using flow cytometry.. In their natural whole-blood milieu, a CB2 receptor-dependent stimulation of the PMN respiratory burst was found at nanomolar concentrations of CP55 940 [( )-cis-3-[2-hydroxy-4-(1,1-dimethylheptyl)phenyl]-trans4-(3-hydroxypropyl)cyclohexanol] and methanandamide after a 3-h incubation period, whereas the short-living and rapidly hydrolyzed endogenous ligand anandamide did not alter the burst reaction of whole-blood PMNs under the same experimental conditions.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: AEA (Cayman Chemical), and [ 3 H]AEA were dissolved in a 1:1:18 mixture of ethanol/alkamus-620/saline.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: In brief, 300 μg of regional homogenate, 500 μg of fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1- 3 H] (a generous gift from the National Institute on Drug Abuse, Bethesda, MD) were incubated in Tris + 1 mM EDTA, pH 7.6 at 37°C in triplicate for 15 min while shaking.

    Ex Vivo:

    Article Title: Amino acid prodrug of capsaicin improves pharmacokinetic properties in the mouse brain and pancreas.
    Article Snippet: A set of 12 standards was prepared for analysis like the biological samples, with the following concentration ranges: 2–5 000 nM of PGE2 (Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin D2 (PGD2, Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin F2α (PGF2α, Tocris Bioscience, Bristol, UK), 0.1–240 nM of AEA (Cayman Chemical Company, Ann Arbor, MI, USA), 7–17 600 nM of 2-AG (Cayman Chemical Company, Ann Arbor, MI, USA), 0.4–1 000 nM of oleoylethanolamide (OEA, Sigma-Aldrich, Burlington, MA, USA), and 0.4–1 100 nM of palmitoylethanolamide (PEA, Sigma-Aldrich, Burlington, MA, USA).

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: AEA (Cayman Chemical) and [3H]AEA were dissolved in a 1:1:18 mixture of ethanol: emulphor: saline.

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: Briefly, 300 μg of regional homogenate, 500μg fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical, Ann Arbor, MI) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1-3H] (a gift from the National Institute on Drug Abuse) were incubated in Tris + 1mM EDTA pH 7.6 at 37°C in triplicate for fifteen minutes while shaking.

    Article Title: Indirect CB2 receptor and mediator-dependent stimulation of human whole-blood neutrophils by exogenous and endogenous cannabinoids.
    Article Snippet: Immunomodulatory effects of endogenous and exogenous cannabinoids have been investigated in numerous studies, mostly performed with isolated cells or transformed cell lines, but only sparse data exist on human polymorphonuclear neutrophils (PMNs).. We therefore investigated the respiratory burst reaction of human whole-blood PMNs under the influence of cannabinoids using flow cytometry.. In their natural whole-blood milieu, a CB2 receptor-dependent stimulation of the PMN respiratory burst was found at nanomolar concentrations of CP55 940 [( )-cis-3-[2-hydroxy-4-(1,1-dimethylheptyl)phenyl]-trans4-(3-hydroxypropyl)cyclohexanol] and methanandamide after a 3-h incubation period, whereas the short-living and rapidly hydrolyzed endogenous ligand anandamide did not alter the burst reaction of whole-blood PMNs under the same experimental conditions.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: AEA (Cayman Chemical), and [ 3 H]AEA were dissolved in a 1:1:18 mixture of ethanol/alkamus-620/saline.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: In brief, 300 μg of regional homogenate, 500 μg of fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1- 3 H] (a generous gift from the National Institute on Drug Abuse, Bethesda, MD) were incubated in Tris + 1 mM EDTA, pH 7.6 at 37°C in triplicate for 15 min while shaking.

    Autoradiography:

    Article Title: Amino acid prodrug of capsaicin improves pharmacokinetic properties in the mouse brain and pancreas.
    Article Snippet: A set of 12 standards was prepared for analysis like the biological samples, with the following concentration ranges: 2–5 000 nM of PGE2 (Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin D2 (PGD2, Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin F2α (PGF2α, Tocris Bioscience, Bristol, UK), 0.1–240 nM of AEA (Cayman Chemical Company, Ann Arbor, MI, USA), 7–17 600 nM of 2-AG (Cayman Chemical Company, Ann Arbor, MI, USA), 0.4–1 000 nM of oleoylethanolamide (OEA, Sigma-Aldrich, Burlington, MA, USA), and 0.4–1 100 nM of palmitoylethanolamide (PEA, Sigma-Aldrich, Burlington, MA, USA).

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: AEA (Cayman Chemical) and [3H]AEA were dissolved in a 1:1:18 mixture of ethanol: emulphor: saline.

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: Briefly, 300 μg of regional homogenate, 500μg fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical, Ann Arbor, MI) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1-3H] (a gift from the National Institute on Drug Abuse) were incubated in Tris + 1mM EDTA pH 7.6 at 37°C in triplicate for fifteen minutes while shaking.

    Article Title: Indirect CB2 receptor and mediator-dependent stimulation of human whole-blood neutrophils by exogenous and endogenous cannabinoids.
    Article Snippet: Immunomodulatory effects of endogenous and exogenous cannabinoids have been investigated in numerous studies, mostly performed with isolated cells or transformed cell lines, but only sparse data exist on human polymorphonuclear neutrophils (PMNs).. We therefore investigated the respiratory burst reaction of human whole-blood PMNs under the influence of cannabinoids using flow cytometry.. In their natural whole-blood milieu, a CB2 receptor-dependent stimulation of the PMN respiratory burst was found at nanomolar concentrations of CP55 940 [( )-cis-3-[2-hydroxy-4-(1,1-dimethylheptyl)phenyl]-trans4-(3-hydroxypropyl)cyclohexanol] and methanandamide after a 3-h incubation period, whereas the short-living and rapidly hydrolyzed endogenous ligand anandamide did not alter the burst reaction of whole-blood PMNs under the same experimental conditions.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: AEA (Cayman Chemical), and [ 3 H]AEA were dissolved in a 1:1:18 mixture of ethanol/alkamus-620/saline.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: In brief, 300 μg of regional homogenate, 500 μg of fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1- 3 H] (a generous gift from the National Institute on Drug Abuse, Bethesda, MD) were incubated in Tris + 1 mM EDTA, pH 7.6 at 37°C in triplicate for 15 min while shaking.

    Modification:

    Article Title: Amino acid prodrug of capsaicin improves pharmacokinetic properties in the mouse brain and pancreas.
    Article Snippet: A set of 12 standards was prepared for analysis like the biological samples, with the following concentration ranges: 2–5 000 nM of PGE2 (Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin D2 (PGD2, Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin F2α (PGF2α, Tocris Bioscience, Bristol, UK), 0.1–240 nM of AEA (Cayman Chemical Company, Ann Arbor, MI, USA), 7–17 600 nM of 2-AG (Cayman Chemical Company, Ann Arbor, MI, USA), 0.4–1 000 nM of oleoylethanolamide (OEA, Sigma-Aldrich, Burlington, MA, USA), and 0.4–1 100 nM of palmitoylethanolamide (PEA, Sigma-Aldrich, Burlington, MA, USA).

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: AEA (Cayman Chemical) and [3H]AEA were dissolved in a 1:1:18 mixture of ethanol: emulphor: saline.

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: Briefly, 300 μg of regional homogenate, 500μg fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical, Ann Arbor, MI) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1-3H] (a gift from the National Institute on Drug Abuse) were incubated in Tris + 1mM EDTA pH 7.6 at 37°C in triplicate for fifteen minutes while shaking.

    Article Title: Indirect CB2 receptor and mediator-dependent stimulation of human whole-blood neutrophils by exogenous and endogenous cannabinoids.
    Article Snippet: Immunomodulatory effects of endogenous and exogenous cannabinoids have been investigated in numerous studies, mostly performed with isolated cells or transformed cell lines, but only sparse data exist on human polymorphonuclear neutrophils (PMNs).. We therefore investigated the respiratory burst reaction of human whole-blood PMNs under the influence of cannabinoids using flow cytometry.. In their natural whole-blood milieu, a CB2 receptor-dependent stimulation of the PMN respiratory burst was found at nanomolar concentrations of CP55 940 [( )-cis-3-[2-hydroxy-4-(1,1-dimethylheptyl)phenyl]-trans4-(3-hydroxypropyl)cyclohexanol] and methanandamide after a 3-h incubation period, whereas the short-living and rapidly hydrolyzed endogenous ligand anandamide did not alter the burst reaction of whole-blood PMNs under the same experimental conditions.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: AEA (Cayman Chemical), and [ 3 H]AEA were dissolved in a 1:1:18 mixture of ethanol/alkamus-620/saline.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: In brief, 300 μg of regional homogenate, 500 μg of fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1- 3 H] (a generous gift from the National Institute on Drug Abuse, Bethesda, MD) were incubated in Tris + 1 mM EDTA, pH 7.6 at 37°C in triplicate for 15 min while shaking.

    Isolation:

    Article Title: Amino acid prodrug of capsaicin improves pharmacokinetic properties in the mouse brain and pancreas.
    Article Snippet: A set of 12 standards was prepared for analysis like the biological samples, with the following concentration ranges: 2–5 000 nM of PGE2 (Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin D2 (PGD2, Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin F2α (PGF2α, Tocris Bioscience, Bristol, UK), 0.1–240 nM of AEA (Cayman Chemical Company, Ann Arbor, MI, USA), 7–17 600 nM of 2-AG (Cayman Chemical Company, Ann Arbor, MI, USA), 0.4–1 000 nM of oleoylethanolamide (OEA, Sigma-Aldrich, Burlington, MA, USA), and 0.4–1 100 nM of palmitoylethanolamide (PEA, Sigma-Aldrich, Burlington, MA, USA).

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: AEA (Cayman Chemical) and [3H]AEA were dissolved in a 1:1:18 mixture of ethanol: emulphor: saline.

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: Briefly, 300 μg of regional homogenate, 500μg fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical, Ann Arbor, MI) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1-3H] (a gift from the National Institute on Drug Abuse) were incubated in Tris + 1mM EDTA pH 7.6 at 37°C in triplicate for fifteen minutes while shaking.

    Article Title: Indirect CB2 receptor and mediator-dependent stimulation of human whole-blood neutrophils by exogenous and endogenous cannabinoids.
    Article Snippet: Immunomodulatory effects of endogenous and exogenous cannabinoids have been investigated in numerous studies, mostly performed with isolated cells or transformed cell lines, but only sparse data exist on human polymorphonuclear neutrophils (PMNs).. We therefore investigated the respiratory burst reaction of human whole-blood PMNs under the influence of cannabinoids using flow cytometry.. In their natural whole-blood milieu, a CB2 receptor-dependent stimulation of the PMN respiratory burst was found at nanomolar concentrations of CP55 940 [( )-cis-3-[2-hydroxy-4-(1,1-dimethylheptyl)phenyl]-trans4-(3-hydroxypropyl)cyclohexanol] and methanandamide after a 3-h incubation period, whereas the short-living and rapidly hydrolyzed endogenous ligand anandamide did not alter the burst reaction of whole-blood PMNs under the same experimental conditions.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: AEA (Cayman Chemical), and [ 3 H]AEA were dissolved in a 1:1:18 mixture of ethanol/alkamus-620/saline.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: In brief, 300 μg of regional homogenate, 500 μg of fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1- 3 H] (a generous gift from the National Institute on Drug Abuse, Bethesda, MD) were incubated in Tris + 1 mM EDTA, pH 7.6 at 37°C in triplicate for 15 min while shaking.

    Imaging:

    Article Title: Amino acid prodrug of capsaicin improves pharmacokinetic properties in the mouse brain and pancreas.
    Article Snippet: A set of 12 standards was prepared for analysis like the biological samples, with the following concentration ranges: 2–5 000 nM of PGE2 (Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin D2 (PGD2, Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin F2α (PGF2α, Tocris Bioscience, Bristol, UK), 0.1–240 nM of AEA (Cayman Chemical Company, Ann Arbor, MI, USA), 7–17 600 nM of 2-AG (Cayman Chemical Company, Ann Arbor, MI, USA), 0.4–1 000 nM of oleoylethanolamide (OEA, Sigma-Aldrich, Burlington, MA, USA), and 0.4–1 100 nM of palmitoylethanolamide (PEA, Sigma-Aldrich, Burlington, MA, USA).

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: AEA (Cayman Chemical) and [3H]AEA were dissolved in a 1:1:18 mixture of ethanol: emulphor: saline.

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: Briefly, 300 μg of regional homogenate, 500μg fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical, Ann Arbor, MI) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1-3H] (a gift from the National Institute on Drug Abuse) were incubated in Tris + 1mM EDTA pH 7.6 at 37°C in triplicate for fifteen minutes while shaking.

    Article Title: Indirect CB2 receptor and mediator-dependent stimulation of human whole-blood neutrophils by exogenous and endogenous cannabinoids.
    Article Snippet: Immunomodulatory effects of endogenous and exogenous cannabinoids have been investigated in numerous studies, mostly performed with isolated cells or transformed cell lines, but only sparse data exist on human polymorphonuclear neutrophils (PMNs).. We therefore investigated the respiratory burst reaction of human whole-blood PMNs under the influence of cannabinoids using flow cytometry.. In their natural whole-blood milieu, a CB2 receptor-dependent stimulation of the PMN respiratory burst was found at nanomolar concentrations of CP55 940 [( )-cis-3-[2-hydroxy-4-(1,1-dimethylheptyl)phenyl]-trans4-(3-hydroxypropyl)cyclohexanol] and methanandamide after a 3-h incubation period, whereas the short-living and rapidly hydrolyzed endogenous ligand anandamide did not alter the burst reaction of whole-blood PMNs under the same experimental conditions.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: AEA (Cayman Chemical), and [ 3 H]AEA were dissolved in a 1:1:18 mixture of ethanol/alkamus-620/saline.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: In brief, 300 μg of regional homogenate, 500 μg of fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1- 3 H] (a generous gift from the National Institute on Drug Abuse, Bethesda, MD) were incubated in Tris + 1 mM EDTA, pH 7.6 at 37°C in triplicate for 15 min while shaking.

    Activity Assay:

    Article Title: Amino acid prodrug of capsaicin improves pharmacokinetic properties in the mouse brain and pancreas.
    Article Snippet: A set of 12 standards was prepared for analysis like the biological samples, with the following concentration ranges: 2–5 000 nM of PGE2 (Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin D2 (PGD2, Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin F2α (PGF2α, Tocris Bioscience, Bristol, UK), 0.1–240 nM of AEA (Cayman Chemical Company, Ann Arbor, MI, USA), 7–17 600 nM of 2-AG (Cayman Chemical Company, Ann Arbor, MI, USA), 0.4–1 000 nM of oleoylethanolamide (OEA, Sigma-Aldrich, Burlington, MA, USA), and 0.4–1 100 nM of palmitoylethanolamide (PEA, Sigma-Aldrich, Burlington, MA, USA).

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: AEA (Cayman Chemical) and [3H]AEA were dissolved in a 1:1:18 mixture of ethanol: emulphor: saline.

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: Briefly, 300 μg of regional homogenate, 500μg fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical, Ann Arbor, MI) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1-3H] (a gift from the National Institute on Drug Abuse) were incubated in Tris + 1mM EDTA pH 7.6 at 37°C in triplicate for fifteen minutes while shaking.

    Article Title: Indirect CB2 receptor and mediator-dependent stimulation of human whole-blood neutrophils by exogenous and endogenous cannabinoids.
    Article Snippet: Immunomodulatory effects of endogenous and exogenous cannabinoids have been investigated in numerous studies, mostly performed with isolated cells or transformed cell lines, but only sparse data exist on human polymorphonuclear neutrophils (PMNs).. We therefore investigated the respiratory burst reaction of human whole-blood PMNs under the influence of cannabinoids using flow cytometry.. In their natural whole-blood milieu, a CB2 receptor-dependent stimulation of the PMN respiratory burst was found at nanomolar concentrations of CP55 940 [( )-cis-3-[2-hydroxy-4-(1,1-dimethylheptyl)phenyl]-trans4-(3-hydroxypropyl)cyclohexanol] and methanandamide after a 3-h incubation period, whereas the short-living and rapidly hydrolyzed endogenous ligand anandamide did not alter the burst reaction of whole-blood PMNs under the same experimental conditions.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: AEA (Cayman Chemical), and [ 3 H]AEA were dissolved in a 1:1:18 mixture of ethanol/alkamus-620/saline.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: In brief, 300 μg of regional homogenate, 500 μg of fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1- 3 H] (a generous gift from the National Institute on Drug Abuse, Bethesda, MD) were incubated in Tris + 1 mM EDTA, pH 7.6 at 37°C in triplicate for 15 min while shaking.

    Software:

    Article Title: Amino acid prodrug of capsaicin improves pharmacokinetic properties in the mouse brain and pancreas.
    Article Snippet: A set of 12 standards was prepared for analysis like the biological samples, with the following concentration ranges: 2–5 000 nM of PGE2 (Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin D2 (PGD2, Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin F2α (PGF2α, Tocris Bioscience, Bristol, UK), 0.1–240 nM of AEA (Cayman Chemical Company, Ann Arbor, MI, USA), 7–17 600 nM of 2-AG (Cayman Chemical Company, Ann Arbor, MI, USA), 0.4–1 000 nM of oleoylethanolamide (OEA, Sigma-Aldrich, Burlington, MA, USA), and 0.4–1 100 nM of palmitoylethanolamide (PEA, Sigma-Aldrich, Burlington, MA, USA).

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: AEA (Cayman Chemical) and [3H]AEA were dissolved in a 1:1:18 mixture of ethanol: emulphor: saline.

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: Briefly, 300 μg of regional homogenate, 500μg fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical, Ann Arbor, MI) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1-3H] (a gift from the National Institute on Drug Abuse) were incubated in Tris + 1mM EDTA pH 7.6 at 37°C in triplicate for fifteen minutes while shaking.

    Article Title: Indirect CB2 receptor and mediator-dependent stimulation of human whole-blood neutrophils by exogenous and endogenous cannabinoids.
    Article Snippet: Immunomodulatory effects of endogenous and exogenous cannabinoids have been investigated in numerous studies, mostly performed with isolated cells or transformed cell lines, but only sparse data exist on human polymorphonuclear neutrophils (PMNs).. We therefore investigated the respiratory burst reaction of human whole-blood PMNs under the influence of cannabinoids using flow cytometry.. In their natural whole-blood milieu, a CB2 receptor-dependent stimulation of the PMN respiratory burst was found at nanomolar concentrations of CP55 940 [( )-cis-3-[2-hydroxy-4-(1,1-dimethylheptyl)phenyl]-trans4-(3-hydroxypropyl)cyclohexanol] and methanandamide after a 3-h incubation period, whereas the short-living and rapidly hydrolyzed endogenous ligand anandamide did not alter the burst reaction of whole-blood PMNs under the same experimental conditions.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: AEA (Cayman Chemical), and [ 3 H]AEA were dissolved in a 1:1:18 mixture of ethanol/alkamus-620/saline.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: In brief, 300 μg of regional homogenate, 500 μg of fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1- 3 H] (a generous gift from the National Institute on Drug Abuse, Bethesda, MD) were incubated in Tris + 1 mM EDTA, pH 7.6 at 37°C in triplicate for 15 min while shaking.

    Generated:

    Article Title: Amino acid prodrug of capsaicin improves pharmacokinetic properties in the mouse brain and pancreas.
    Article Snippet: A set of 12 standards was prepared for analysis like the biological samples, with the following concentration ranges: 2–5 000 nM of PGE2 (Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin D2 (PGD2, Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin F2α (PGF2α, Tocris Bioscience, Bristol, UK), 0.1–240 nM of AEA (Cayman Chemical Company, Ann Arbor, MI, USA), 7–17 600 nM of 2-AG (Cayman Chemical Company, Ann Arbor, MI, USA), 0.4–1 000 nM of oleoylethanolamide (OEA, Sigma-Aldrich, Burlington, MA, USA), and 0.4–1 100 nM of palmitoylethanolamide (PEA, Sigma-Aldrich, Burlington, MA, USA).

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: AEA (Cayman Chemical) and [3H]AEA were dissolved in a 1:1:18 mixture of ethanol: emulphor: saline.

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: Briefly, 300 μg of regional homogenate, 500μg fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical, Ann Arbor, MI) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1-3H] (a gift from the National Institute on Drug Abuse) were incubated in Tris + 1mM EDTA pH 7.6 at 37°C in triplicate for fifteen minutes while shaking.

    Article Title: Indirect CB2 receptor and mediator-dependent stimulation of human whole-blood neutrophils by exogenous and endogenous cannabinoids.
    Article Snippet: Immunomodulatory effects of endogenous and exogenous cannabinoids have been investigated in numerous studies, mostly performed with isolated cells or transformed cell lines, but only sparse data exist on human polymorphonuclear neutrophils (PMNs).. We therefore investigated the respiratory burst reaction of human whole-blood PMNs under the influence of cannabinoids using flow cytometry.. In their natural whole-blood milieu, a CB2 receptor-dependent stimulation of the PMN respiratory burst was found at nanomolar concentrations of CP55 940 [( )-cis-3-[2-hydroxy-4-(1,1-dimethylheptyl)phenyl]-trans4-(3-hydroxypropyl)cyclohexanol] and methanandamide after a 3-h incubation period, whereas the short-living and rapidly hydrolyzed endogenous ligand anandamide did not alter the burst reaction of whole-blood PMNs under the same experimental conditions.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: AEA (Cayman Chemical), and [ 3 H]AEA were dissolved in a 1:1:18 mixture of ethanol/alkamus-620/saline.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: In brief, 300 μg of regional homogenate, 500 μg of fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1- 3 H] (a generous gift from the National Institute on Drug Abuse, Bethesda, MD) were incubated in Tris + 1 mM EDTA, pH 7.6 at 37°C in triplicate for 15 min while shaking.

    In Vitro:

    Article Title: Amino acid prodrug of capsaicin improves pharmacokinetic properties in the mouse brain and pancreas.
    Article Snippet: A set of 12 standards was prepared for analysis like the biological samples, with the following concentration ranges: 2–5 000 nM of PGE2 (Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin D2 (PGD2, Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin F2α (PGF2α, Tocris Bioscience, Bristol, UK), 0.1–240 nM of AEA (Cayman Chemical Company, Ann Arbor, MI, USA), 7–17 600 nM of 2-AG (Cayman Chemical Company, Ann Arbor, MI, USA), 0.4–1 000 nM of oleoylethanolamide (OEA, Sigma-Aldrich, Burlington, MA, USA), and 0.4–1 100 nM of palmitoylethanolamide (PEA, Sigma-Aldrich, Burlington, MA, USA).

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: AEA (Cayman Chemical) and [3H]AEA were dissolved in a 1:1:18 mixture of ethanol: emulphor: saline.

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: Briefly, 300 μg of regional homogenate, 500μg fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical, Ann Arbor, MI) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1-3H] (a gift from the National Institute on Drug Abuse) were incubated in Tris + 1mM EDTA pH 7.6 at 37°C in triplicate for fifteen minutes while shaking.

    Article Title: Indirect CB2 receptor and mediator-dependent stimulation of human whole-blood neutrophils by exogenous and endogenous cannabinoids.
    Article Snippet: Immunomodulatory effects of endogenous and exogenous cannabinoids have been investigated in numerous studies, mostly performed with isolated cells or transformed cell lines, but only sparse data exist on human polymorphonuclear neutrophils (PMNs).. We therefore investigated the respiratory burst reaction of human whole-blood PMNs under the influence of cannabinoids using flow cytometry.. In their natural whole-blood milieu, a CB2 receptor-dependent stimulation of the PMN respiratory burst was found at nanomolar concentrations of CP55 940 [( )-cis-3-[2-hydroxy-4-(1,1-dimethylheptyl)phenyl]-trans4-(3-hydroxypropyl)cyclohexanol] and methanandamide after a 3-h incubation period, whereas the short-living and rapidly hydrolyzed endogenous ligand anandamide did not alter the burst reaction of whole-blood PMNs under the same experimental conditions.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: AEA (Cayman Chemical), and [ 3 H]AEA were dissolved in a 1:1:18 mixture of ethanol/alkamus-620/saline.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: In brief, 300 μg of regional homogenate, 500 μg of fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1- 3 H] (a generous gift from the National Institute on Drug Abuse, Bethesda, MD) were incubated in Tris + 1 mM EDTA, pH 7.6 at 37°C in triplicate for 15 min while shaking.

    Membrane:

    Article Title: Amino acid prodrug of capsaicin improves pharmacokinetic properties in the mouse brain and pancreas.
    Article Snippet: A set of 12 standards was prepared for analysis like the biological samples, with the following concentration ranges: 2–5 000 nM of PGE2 (Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin D2 (PGD2, Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin F2α (PGF2α, Tocris Bioscience, Bristol, UK), 0.1–240 nM of AEA (Cayman Chemical Company, Ann Arbor, MI, USA), 7–17 600 nM of 2-AG (Cayman Chemical Company, Ann Arbor, MI, USA), 0.4–1 000 nM of oleoylethanolamide (OEA, Sigma-Aldrich, Burlington, MA, USA), and 0.4–1 100 nM of palmitoylethanolamide (PEA, Sigma-Aldrich, Burlington, MA, USA).

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: AEA (Cayman Chemical) and [3H]AEA were dissolved in a 1:1:18 mixture of ethanol: emulphor: saline.

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: Briefly, 300 μg of regional homogenate, 500μg fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical, Ann Arbor, MI) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1-3H] (a gift from the National Institute on Drug Abuse) were incubated in Tris + 1mM EDTA pH 7.6 at 37°C in triplicate for fifteen minutes while shaking.

    Article Title: Indirect CB2 receptor and mediator-dependent stimulation of human whole-blood neutrophils by exogenous and endogenous cannabinoids.
    Article Snippet: Immunomodulatory effects of endogenous and exogenous cannabinoids have been investigated in numerous studies, mostly performed with isolated cells or transformed cell lines, but only sparse data exist on human polymorphonuclear neutrophils (PMNs).. We therefore investigated the respiratory burst reaction of human whole-blood PMNs under the influence of cannabinoids using flow cytometry.. In their natural whole-blood milieu, a CB2 receptor-dependent stimulation of the PMN respiratory burst was found at nanomolar concentrations of CP55 940 [( )-cis-3-[2-hydroxy-4-(1,1-dimethylheptyl)phenyl]-trans4-(3-hydroxypropyl)cyclohexanol] and methanandamide after a 3-h incubation period, whereas the short-living and rapidly hydrolyzed endogenous ligand anandamide did not alter the burst reaction of whole-blood PMNs under the same experimental conditions.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: AEA (Cayman Chemical), and [ 3 H]AEA were dissolved in a 1:1:18 mixture of ethanol/alkamus-620/saline.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: In brief, 300 μg of regional homogenate, 500 μg of fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1- 3 H] (a generous gift from the National Institute on Drug Abuse, Bethesda, MD) were incubated in Tris + 1 mM EDTA, pH 7.6 at 37°C in triplicate for 15 min while shaking.

    Saline:

    Article Title: Amino acid prodrug of capsaicin improves pharmacokinetic properties in the mouse brain and pancreas.
    Article Snippet: A set of 12 standards was prepared for analysis like the biological samples, with the following concentration ranges: 2–5 000 nM of PGE2 (Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin D2 (PGD2, Tocris Bioscience, Bristol, UK), 2–5 000 nM of prostaglandin F2α (PGF2α, Tocris Bioscience, Bristol, UK), 0.1–240 nM of AEA (Cayman Chemical Company, Ann Arbor, MI, USA), 7–17 600 nM of 2-AG (Cayman Chemical Company, Ann Arbor, MI, USA), 0.4–1 000 nM of oleoylethanolamide (OEA, Sigma-Aldrich, Burlington, MA, USA), and 0.4–1 100 nM of palmitoylethanolamide (PEA, Sigma-Aldrich, Burlington, MA, USA).

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: AEA (Cayman Chemical) and [3H]AEA were dissolved in a 1:1:18 mixture of ethanol: emulphor: saline.

    Article Title: Temporal changes in mouse brain fatty acid amide hydrolase activity
    Article Snippet: Briefly, 300 μg of regional homogenate, 500μg fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical, Ann Arbor, MI) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1-3H] (a gift from the National Institute on Drug Abuse) were incubated in Tris + 1mM EDTA pH 7.6 at 37°C in triplicate for fifteen minutes while shaking.

    Article Title: Indirect CB2 receptor and mediator-dependent stimulation of human whole-blood neutrophils by exogenous and endogenous cannabinoids.
    Article Snippet: Immunomodulatory effects of endogenous and exogenous cannabinoids have been investigated in numerous studies, mostly performed with isolated cells or transformed cell lines, but only sparse data exist on human polymorphonuclear neutrophils (PMNs).. We therefore investigated the respiratory burst reaction of human whole-blood PMNs under the influence of cannabinoids using flow cytometry.. In their natural whole-blood milieu, a CB2 receptor-dependent stimulation of the PMN respiratory burst was found at nanomolar concentrations of CP55 940 [( )-cis-3-[2-hydroxy-4-(1,1-dimethylheptyl)phenyl]-trans4-(3-hydroxypropyl)cyclohexanol] and methanandamide after a 3-h incubation period, whereas the short-living and rapidly hydrolyzed endogenous ligand anandamide did not alter the burst reaction of whole-blood PMNs under the same experimental conditions.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: AEA (Cayman Chemical), and [ 3 H]AEA were dissolved in a 1:1:18 mixture of ethanol/alkamus-620/saline.

    Article Title: Cyclooxygenase-2 Mediates Anandamide Metabolism in the Mouse Brain
    Article Snippet: In brief, 300 μg of regional homogenate, 500 μg of fatty acid-free bovine serum albumin, 100 μM AEA (Cayman Chemical) plus 0.22 μCi arachidonoyl ethanolamide [ethanolamine-1- 3 H] (a generous gift from the National Institute on Drug Abuse, Bethesda, MD) were incubated in Tris + 1 mM EDTA, pH 7.6 at 37°C in triplicate for 15 min while shaking.



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    Acute treatment with anandamide (AEA) does not affect blood pressure in Dahl SS rats on a normal salt diet. Mean arterial pressure (MAP) in male SS rats after a single injection of AEA at a dose 0.05 mg/kg ( A, B ) and 3 mg/kg ( C, D ) or a corresponding vehicle in SS rats on a normal salt (NS, 0.4% NaCl) diet. ( B, D ) Summary graphs of average MAP 2 h after AEA administration in a low ( B ) or a high ( D ) doses.

    Journal: Scientific Reports

    Article Title: Acute and long-term effects of cannabinoids on hypertension and kidney injury

    doi: 10.1038/s41598-022-09902-6

    Figure Lengend Snippet: Acute treatment with anandamide (AEA) does not affect blood pressure in Dahl SS rats on a normal salt diet. Mean arterial pressure (MAP) in male SS rats after a single injection of AEA at a dose 0.05 mg/kg ( A, B ) and 3 mg/kg ( C, D ) or a corresponding vehicle in SS rats on a normal salt (NS, 0.4% NaCl) diet. ( B, D ) Summary graphs of average MAP 2 h after AEA administration in a low ( B ) or a high ( D ) doses.

    Article Snippet: Anandamide (AEA, Cayman chemical, #90,050) was used at a dose 0.05 mg/kg (low dose) or 3 mg/kg (high dose).

    Techniques: Injection